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Peroxidase and laccase as catalysts for removal of the phenylhydrazide protecting group under mild conditions
Author(s) -
Semenov Anatoly N.,
Lomonsova Irina V.,
Berezin Vasilly I.,
Titov Mikhail I.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260421002
Subject(s) - laccase , peroxidase , chemistry , catalysis , enzyme , organic chemistry
A new method is proposed for the removal of the phenylhydrazide protecting group by the action of peroxidase or laccase, the enzymes attributed to the class of oxidoreductases. The deblocking procedure is performed under mild oxidative conditions, i.e., aqueous solution and neutral or close to neutral pH. Such mild oxidizing agents as 1 m M H 2 O 2 and air oxygen are used for unmasking. The method is available for the deblocking of both α‐ and γ‐carboxyl groups. The enzyme‐catalyzed removal of the phenylhydrazide protecting group causes no oxidative modification nor destruction of methionine or tryptophan side chains. © 1993 John Wiley & Sons, Inc.