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Production of Bacillus thuringiensis spores in total cell retention culture and two‐stage continuous culture using an internal ceramic filter system
Author(s) -
Kang Byung Chul,
Lee Sang Yup,
Chang Ho Nam
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420913
Subject(s) - spore , bacillus thuringiensis , bioreactor , dilution , chromatography , biology , chemistry , food science , botany , bacteria , genetics , physics , thermodynamics
The production of Bacillus thuringiensis spores was investigated in a bioreactor incorporating a ceramic membrane filter to improve spore concentration and volumetric productivity. Two cultivation methods were used in this study: a total cell retention culture (TCRC), and a two‐stage continuous culture with partial cell bleeding. In the TCRC, fed by 50 g/L of glucose, a spore concentration of 1.6 × 10 10 CFU/mL was obtained with a spore percentage of greater than 95% and a maximum cell mass of 82.2 g/L. The volumetric productivity was four times higher than that obtained from batch cultivation. In the two‐stage continuous culture with partial cell bleeding spore concentration was strongly dependent on the bleed ratio. The spore concentration of 1.8 × 10 9 CFU/mL and the spore percentage of 70% were obtained at the second stage when a bleed ratio of 0.33 and a dilution rate of 0.23 h −1 were used. © 1993 John Wiley & Sons, Inc.