Premium
Protein adsorption in polysulfone hollow fiber bioreactors used for serum‐free mammalian cell culture
Author(s) -
Patkar Anant Y.,
Bowen Bruce D.,
Piret James M.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420912
Subject(s) - transferrin , adsorption , bovine serum albumin , chromatography , chemistry , albumin , serum albumin , ultrafiltration (renal) , polysulfone , bioreactor , fiber , membrane , blood proteins , protein adsorption , biochemistry , organic chemistry
The recovery of serum‐free medium proteins from poly‐sulfone hollow fiber bioreactors (HFBRs) was investigated. More than 99% of the initial transferrin was adsorbed to the hydrophobic hollow fibers within 2 h of HFBR operation. A methodology to minimize transferrin adsorption by pre‐adsorption of bovine serum albumin (BSA) was developed. BSA adsorption on suspended cut fibers was virtually complete within 1 h. BSA‐coated fibers adsorbed only 5% of the transferrin within 10 days, whereas uncoated cut fibers adsorbed more than 99% of the transferrin within 1 h. An improved HFBR startup procedure, using a BSA‐coating step before inoculation, resulted in substantially higher transferrin recovery. Additional factors influenced extracapillary space (ECS) transferrin concentrations. Pronounced downstream polarization of transferrin was observed in the ECS. In addition, the 30‐kDa nominal molecular weight cutoff ultrafiltration membranes rapidly leaked transferrin from the ECS to the lumen. © 1993 John Wiley & Sons, Inc.