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Lipase‐Catalyzed acyiation of sugars solubilized in hydrophobic solvents by complexation
Author(s) -
Ikeda Isao,
Klibanov Alexander M.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420616
Subject(s) - lipase , solubilization , chemistry , catalysis , organic chemistry , triacylglycerol lipase , chromatography , enzyme , biochemistry
By complexing glucose and other mono‐ and disaccharides with phenylboronic acid, they were solubilized in many organic solvents. Pseudomonas sp. lipoprotein lipase readily acylated such solubilized sugars in these solvents (no reaction was observed without phenylboronic acid, i.e., when sugars are insoluble in the reaction medium). Solubilized D‐glucose was enzymatically acylated with vinyl acrylate on a preparative scale, and the resultant 6‐ O ‐acryloylglucose was chemically polymerized to yield a sugar‐based polyacrylate with a molecular weight of 14,000 Da. © 1993 John Wiley & Sons, Inc.