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Modeling lipolysis in a reversed micellar system: Part II—membrane reactor
Author(s) -
Prazeres D. M. F.,
Lemos F.,
Garcia F. A. P.,
Cabral J. M. S.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420612
Subject(s) - membrane reactor , chemistry , hydrolysis , substrate (aquarium) , membrane , chromatography , bioreactor , product inhibition , lipolysis , membrane bioreactor , lipase , batch reactor , continuous stirred tank reactor , chemical engineering , organic chemistry , biochemistry , enzyme , catalysis , oceanography , non competitive inhibition , adipose tissue , engineering , geology
Olive oil hydrolysis using Chromobacterium viscosum lipase B in a reversed micellar media was investigated in a membrane reactor. The dynamic evolution of the product concentration both in the concentrate and permeate stream was analyzed using a mechanistic model previously developed by us and further modified in this work. A kinetic law with a second‐order dependence in the substrate concentration and nonlinear product inhibition was found to be the most adequate for the description of the hydrolysis data over an extensive range of time and substrate concentration. These findings are discussed in terms of the specific interactions occurring in the membrane reactor. © 1993 John Wiley & Sons, Inc.