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Carbon and energy balances in cell‐recycle cultures of Schizosaccharomyces pombe
Author(s) -
Uribelarrea J. L.,
De Queiroz H.,
Goma G.,
Pareilleux A.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420608
Subject(s) - schizosaccharomyces pombe , catabolism , yeast , fermentation , schizosaccharomyces , oxidative phosphorylation , biochemistry , chemistry , carbon fibers , steady state (chemistry) , yield (engineering) , fed batch culture , fission , carbon source , metabolism , food science , saccharomyces cerevisiae , thermodynamics , materials science , organic chemistry , composite number , composite material , physics , quantum mechanics , neutron
A strain of the fission yeast Schizosaccharomyces pombe was aerobically grown in a cell‐recycle fermentor under various operating conditions, i.e., different bleeding rates and various separate feed rates of glucose and basal medium. Carbon and energy balances were analyzed during steady‐state culture regimes, allowing growth yields and maintenance coefficients to be determined under glucose‐limited and glucose‐excess environments. Special attention was given to the metabolic shift from purely oxidative to respirofermentative glucose catabolism resulting from a change in the growth‐limiting factor. No maintenance requirements for the carbon source and for energy were observed during glucose‐limited culture regimes and oxidative catabolism. Under glucose excess and respirofermentative metabolism, the m G coefficient was shown to be growth‐linked, whereas the enhancement of the apparent m e coefficient observed for increased residual glucose concentrations could be assigned to a decline in the ATP yield. © 1993 John Wiley & Sons, Inc.