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Oxygen transfer in a pulse bioreactor
Author(s) -
Monahan Patrick B.,
Holtzapple Mark T.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420607
Subject(s) - bioreactor , oxygen , agitator , microcarrier , laboratory flask , materials science , chemistry , chromatography , mass transfer , cell , biochemistry , organic chemistry
Oxygen transfer in a novel pulse bioreactor has been evaluated. The agitator consists of a series of alternately fixed and movable parallel plates mounted so that the movable plates vibrate at 30 Hz causing a pulsating fluid motion. Pure oxygen, at pressures up to 5 atm, diffuses through silicone rubber tubing that also vibrates at 30 or 60 Hz. The main feature of this bioreactor is high oxygen transfer with low shear to prevent damage to fragile animal cell membranes. We estimate that sufficient oxygen can be supplied to support over 10 8 cells/mL of human diploid foreskin cells growing on microcarriers. © 1993 John Wiley & Sons, Inc.