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Partitioning behavior and enrichment of proteins with reversed micellar extraction: I. Forward extraction of proteins from aqueous to reversed micellar phase
Author(s) -
Nishiki Tadaaki,
Sato Ikuto,
Kataoka Takeshi,
Kato Daisaburo
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420507
Subject(s) - extraction (chemistry) , chromatography , chemistry , aqueous solution , phase (matter) , aqueous two phase system , micellar solutions , micelle , organic chemistry
Protein extractions using aerosol OT (AOT)–isooctane reverse micelle solutions have been studied to explore the potential for separating and enriching proteins with the reversed micellar extraction. The effects of pH, ionic strength, and different cations of chlorides in a bulk aqueous phase and of AOT concentration in an organic phase on the partitioning of lysozyme and myoglobin and the solubilization of water are presented in detail. The extraction of lysozyme was affected by the concentration of potassium or barium but was almost independent of that of sodium or calcium, whose ionic diameter is smaller than that of potassium and barium. For the extraction of myoglobin, however, the effect of barium concentration was not appreciable. Lysozyme could be enriched into the reversed micellar phase up to 30 times the aqueous feed concentration. © 1993 John Wiley & Sons, Inc.