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Immobilized Coffea arabica cell culture using a bubble‐column reactor with controlled light intensity
Author(s) -
Kurata Hiroyuki,
Furusaki Shintaro
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420413
Subject(s) - light intensity , bioreactor , chromatography , calcium alginate , intensity (physics) , coffea arabica , bubble column reactor , chemistry , bubble , biophysics , chemical engineering , botany , calcium , biology , optics , organic chemistry , physics , parallel computing , gas bubble , computer science , engineering
Coffea arabica cells immobilized by calcium alginate gel were photocultured using a bubble‐column reactor under controlled light intensity. This process was carried out after their alkaloid productivity was improved by increasing the cell density in the initial gel matrix and preculturing the immobilized cells in the dark prior to light irradiation. The cells were grown in the form of a biofilm on gel beads, producing 100 mg/L of purine alkaloids in a 24‐day batch culture. Alkaloid production was relatively constant with respect to light intensity changes, and also cell growth was not suppressed much at high light intensity, with these behaviors being different from those obtained using suspended cells. These phenomena are explained by estimating the light intensity gradient within the cell‐immobilizing particles and by measuring the viable cell distribution within them. It subsequently suggests that the subsurface cells affect both the production and growth behaviors. © 1993 John Wiley & Sons, Inc.