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Bioconversion of lactose/whey to fructose diphosphate with recombinant saccharomyces cerevisiae cells
Author(s) -
Compagno C.,
Tura A.,
Ranzi B. M.,
Martegani E.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420319
Subject(s) - bioconversion , lactose , saccharomyces cerevisiae , fructose , yeast , biochemistry , escherichia coli , chemistry , recombinant dna , substrate (aquarium) , food science , biology , fermentation , gene , ecology
Genetically engineered Saccharomyces cerevisiae strains that express Escherichia coli β‐galactosidase gene are able to bioconvert lactose or whey into fructose‐1,6‐diphosphate (FDP). High FDP yields from whey were obtained with an appropriate ratio between cell concentration and inorganic phosphate. The biomass of transformed cells can be obtained from different carbon sources, according to the expression vector bearing the lacZ gene. We showed that whey can be used as the carbon source for S. cerevisiae growth and as the substrate for bioconversion to fructose diphosphate. © 1993 John Wiley & Sons, Inc.