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Microencapsulation selection for isolation of yeast mutants with increased secretion of Aspergillus awamori glucoamylase
Author(s) -
Wittrup K. D.,
Weber A. L.,
Tsai P.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420312
Subject(s) - aspergillus awamori , yeast , isolation (microbiology) , mutant , aspergillus , secretion , selection (genetic algorithm) , microorganism , biology , enzyme , chemistry , biochemistry , microbiology and biotechnology , bacteria , genetics , gene , computer science , artificial intelligence
We have developed a microencapsulation selection method which allows the rapid and quantitative screening of >10 6 yeast cells for enhanced secretion of Aspergillus awamori glucoamylase. The method provides a 400‐fold single‐pass enrichment for high‐secreting mutants, and can be straightforwardly adapted for application to growth‐based selection schemes with other microorganisms and enzymes. © 1993 John Wiley & Sons, Inc.