Microencapsulation selection for isolation of yeast mutants with increased secretion of  Aspergillus awamori  glucoamylase | Zendy

Wittrup K. D. | Zendy; Weber A. L. | Zendy; Tsai P. | Zendy

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Microencapsulation selection for isolation of yeast mutants with increased secretion of Aspergillus awamori glucoamylase

Author(s) -

Wittrup K. D.,

Weber A. L.,

Tsai P.

Publication year - 1993

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260420312

Subject(s) - aspergillus awamori , yeast , isolation (microbiology) , mutant , aspergillus , secretion , selection (genetic algorithm) , microorganism , biology , enzyme , chemistry , biochemistry , microbiology and biotechnology , bacteria , genetics , gene , computer science , artificial intelligence

We have developed a microencapsulation selection method which allows the rapid and quantitative screening of >10 6 yeast cells for enhanced secretion of Aspergillus awamori glucoamylase. The method provides a 400‐fold single‐pass enrichment for high‐secreting mutants, and can be straightforwardly adapted for application to growth‐based selection schemes with other microorganisms and enzymes. © 1993 John Wiley & Sons, Inc.

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