Premium
Loss of antibody productivity is highly reproducible in multiple hybridoma subclones
Author(s) -
Merritt Steven E.,
Palsson Bernhard O.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420213
Subject(s) - antibody , population , biology , productivity , microbiology and biotechnology , phenotype , chemistry , biochemistry , immunology , gene , demography , sociology , economics , macroeconomics
An immunoglobulin G (IgG 2b ) producing hybridoma cell line (S3H5/γ2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum‐free medium. Following adaptation to serum‐free medium, it was found that 16 of the 20 subclones remained at a relatively constant proportion of nonproducing cells. Three of the remaining subclones transiently deviated from this balance but eventually returned toward this population composition. One subclone continued to lose productivity. A population balance was reached at approximately 8% of the population being nonproducers. The loss of antibody productivity was thus highly reproducible. © 1993 John Wiley & Sons, Inc.