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Cell retention–chemostat studies of hybridoma cells—analysis of hybridoma growth and metabolism in continuous suspension culture in serum‐free medium
Author(s) -
Hiller G. W.,
Clark D. S.,
Blanch H. W.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260420206
Subject(s) - chemostat , metabolism , cell culture , perfusion , steady state (chemistry) , suspension culture , oxidative metabolism , cell growth , biochemistry , suspension (topology) , chromatography , biology , oxidative phosphorylation , chemistry , bioreactor , medicine , botany , genetics , mathematics , homotopy , pure mathematics , bacteria
The steady‐state metabolic parameters for a hybridoma cell line have been determined in continuous suspension–perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at high perfusion rates and low cell bleed rates. At the low growth rates examined in this study, cellular metabolism shifted to become more oxidative, and as a result, the fraction of consumed substrate converted to inhibitory metabolic by‐products was reduced. Specific antibody productivity was found to be non–growth associated. © 1993 John Wiley & Sons, Inc.