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Pulsatile flow filtration of yeast cell debris: Influence of preincubation on performance
Author(s) -
Wu D.,
Howell J. A.,
Field R. W.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260411011
Subject(s) - pulsatile flow , microfiltration , yeast , baffle , suspension (topology) , flux (metallurgy) , chromatography , filtration (mathematics) , chemistry , flow (mathematics) , mixing (physics) , vortex , membrane , materials science , mechanics , biophysics , biology , chemical engineering , biochemistry , physics , mathematics , statistics , organic chemistry , quantum mechanics , homotopy , pure mathematics , engineering , endocrinology
Abstract The recovery of proteins from yeast cell debris suspension was investigated using a vortex mixing technique based on the combination of oscillatory flow and a baffled flat‐sheet microfiltration system. For this system, increased protein transmission was obtained through the use of low transmembrane pressures and the preincubation of the yeast cell debris feed suspension at 30°C. Furthermore, a plateau in the flux‐time curve was observed. In the absence of baffles and pulsations, preincubation had a little effect. © 1993 Wiley & Sons, Inc.