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Mechanism of microbial flotation using Thiobacillus ferrooxidans for pyrite suppression
Author(s) -
Ohmura Naoya,
Kitamura Keiko,
Saiki Hiroshi
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260410611
Subject(s) - thiobacillus ferrooxidans , pyrite , thiobacillus , mechanism (biology) , chemistry , environmental chemistry , microbiology and biotechnology , mineralogy , biology , sulfur , organic chemistry , philosophy , epistemology
Microbial desulfurization might be developed as a new process for the removal of pyrite sulfur from coal sluries such as coal–water mixture (CWM). An application of iron‐oxidizing bacterium Thiobacillus ferrooxidans to flotation would shorten the periods of the microbial removal of pyrite from some weeks by leaching methods to a few minutes. The floatability of pyrite in flotation was mainly reduced by T. ferrooxidans itself rather than by other microbial substances in bacterial culture as additive of flotation liquor. Floatability was suppressed within a few seconds by bacterial contact. The suppression was proportional to increasing the number of cells observed between bacterial adhesion and the suppression of floatability. If 25% of the total pyrite surface area covered with the bacteria, pyrite floatability would be completely depressed. Bacteria that lost their iron‐oxidizing activities by sodium cyanide treatment were also able to adhere to pyrite and reduced pyrite floatability as much as normal bacteria did. Thiobacillus ferrooxidans ATCC 23270, T‐1, 9, and 11, which had different iron‐oxidizing abilities, suppressed floatability to similar‐levels. The oxidizing ability of bacteria did not influence the suppressing effect. These results showed the mechanism of the suppression of pyrite floatability by bacteria. Quick bacterial adhesion to pyrite induced floatability suppression by changing the surface property from hydrophobic. The quick adhesion of the bacterium was the novel function which worked to change the surface property of pyrite to remove it from coal. © 1993 John Wiley & Sons, Inc.