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Dynamics of transcriptional and translational processes in hepatocytes cultured in a collagen sandwich
Author(s) -
Dunn James C. Y.,
Tompkins Ronald G.,
Yarmush Martin L.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260410512
Subject(s) - albumin , hepatocyte , messenger rna , in vivo , serum albumin , transcription (linguistics) , protein biosynthesis , biochemistry , chemistry , gene expression , secretion , amino acid , microbiology and biotechnology , biology , biophysics , in vitro , gene , genetics , linguistics , philosophy
A mathematical model which simulates the dynamic behavior of the hepatocytes cultured in a collagen sandwich is presented. Using several independently determined experimental parameters (e.g., albumin gene nuclear runoff activity, the level of albumin mRNA, and the albumin secretion rate), we have used this model to calculate the in vivo albumin gene transcriptional rate (0.27 molecules per second per hepatocyte), the half‐life of albumin mRNA (3.3 days) in cultured hepatocytes, and the albumin polypeptide elongation rate (10 amino acids per second). In addition, the characteristic time constants for the transient increases in transcription (5 days) and in translation (10 days) were also obtained. These bestfit parameters were used to predict the rate of albumin secretion in rescued hepatocytes. © 1993 John Wiley & Sons, Inc.