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Enzymatic activity under tangential flow conditions of photochemically grafted membranes containing immobilized catalase
Author(s) -
Selli Elena,
D'Ambrosio Andrea,
Bellobono Ignazio Renato
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260410411
Subject(s) - membrane , substrate (aquarium) , chemistry , ultrafiltration (renal) , hydrogen peroxide , catalase , chemical engineering , diffusion , cellulose , immobilized enzyme , chromatography , polymer chemistry , enzyme , organic chemistry , biochemistry , oceanography , physics , engineering , thermodynamics , geology
Catalase has been immobilized within sandwich membranes prepared by the photoinduced grafting of an epoxy‐diacrylate prepolymer onto commercial asymmetric cellulose membranes. The enzymatic activity of the membrane composite of hydrogen peroxide decomposition has been studied in a recirculation apparatus under tangential flow conditions without ultrafiltration. The enzymatic membranes were exposed to very low mechanical stresses and showed a very good catalytic performance and durability. Initial reaction rates, measured at 25°C as a function of both substrate concentration and enzyme amount immobilized per unit membrane surface, indicate that the mechanism of action of catalase is not altered after immobilization, although substrate diffusion through the original thin layer of membranes may become rate controlling. © 1993 John Wiley & Sons, Inc.