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Poly(ethylenimine)‐reinforced liquid‐core capsules for the cultivation of hybridoma cells
Author(s) -
Hsu YuanLung,
Chu IMing
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260401103
Subject(s) - capsule , chromatography , aeration , chemistry , solvent , materials science , nuclear chemistry , biochemistry , biology , organic chemistry , botany
The mechanical stability of liquid‐core alginate‐poly‐ L ‐lysine (PLL) capsules used for encapsulation of hybridoma cells can be greatly enhanced by the inclusion of poly(ethylenimine) (PEI) in the hardening solution containing calcium chloride. The PEI can also reinforce the PLL‐coated carboxymethyl‐celluose liquid‐core capsules. The cultivation of murein hybridoma CT04 in these two capsules was carried out. Cell concentrations higher than 10 8 cells/mL per capsule were obtained with ca. 80% of the specific antibody productivity as the freely suspended cells. These capsules could withstand severe agitation and aeration in an air‐lift reactor over a period of 3 weeks with minimal damage. © 1992 John Wiley & Sons, Inc.