Premium
Cell growth on immobilized cell growth factor. 8. Protein‐free cell culture on insulin‐immobilized microcarriers
Author(s) -
Ito Yoshihiro,
Uno Tsuyoshi,
Liu Shu Qin,
Imanishi Yukio
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260401017
Subject(s) - microcarrier , insulin , cell culture , cell , chemistry , cell growth , growth factor , biochemistry , biology , microbiology and biotechnology , endocrinology , genetics , receptor
In order to develop a new protein‐free cell culture system, microcarriers immobilized with insulin were synthesized. For the synthesis, glass and polyacrylamide beads were treated for the introduction of amino groups on the surface, and insulin was immobilized on the surface by using several method. Anchorage‐dependent cells. mouse fibroblast cells STO and fibroic sarcoma cells HSDM 1 C 1 , and the anchorage‐independent cells, mouse hybridoma cells SJK132‐20 and RDP 45/20 were cultivated on the microcarriers immobilized with insulin. The insulin‐immobilized microcarriers did not have any effect on the proliferation of the anchorage independent cells but promoted the growth of anchorage‐dependent cells remarkably. The activity of immobilized insulin was larger than that of free or adsorbed insulin. The repeated use of the insulin‐immobilized microcarrier was possible, and the promotion activity in the the repeated use was greater than that in the use. © 1992 John Wiley & Sons, Inc.