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Kinetic studies on urea hydrolysis by immobilized urease in a batch squeezer and flow reactor
Author(s) -
Huang TingChia,
Chen DongHwang
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260401010
Subject(s) - urease , hydrolysis , polyurethane , bioreactor , urea , immobilized enzyme , chemistry , batch reactor , ammonia volatilization from urea , glutaraldehyde , chemical engineering , continuous reactor , chromatography , amidase , materials science , organic chemistry , enzyme , catalysis , engineering
The immobilization of urease on the reticulated polyurethane foam, and the kinetic phenomenon of urea hydrolysis by the resulting immobilized urease in both batch squeezer and circulated flow reactors were studied. Urease was immobilized with bovine serum albumin and glutaraldehyde on polyurethane foam support of 7 to 15 μm thickness. The residual apparent activity of urease after immobilization was about 50%. The good hydrodynamic property and flexibility of polyurethane foam were retained in solution after immobilization. A modified biofilm reactor model was used to describe the kinetic phenomenon of urea hydrolysis in both batch squeezer and circulated flow reactors. The characteristic parameters of the reactor model for both bioreactors were obtained by combining the Rosenbrock optimization method, the Rungs–Kutta method, and the Newton–Raphson method. The best‐fit results were in good agreement with the experimental data. This study suggests another application of polyurethane foam in enzyme immobilization and immobilized enzyme reactors, which offers potential for practical applications in various bioreactors. © 1992 John Wiley & Sons, Inc.