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Investigation of reduced serum and serum‐free media for the cultivation of insect cells (Bm5) and the production of baculovirus (BmNPV)
Author(s) -
Zhang Junli,
Kalogerakis Nicolas,
Behie Leo A.,
Iatrou Kostas
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260401005
Subject(s) - fetal bovine serum , cell culture , chemistry , cell , bovine serum albumin , microbiology and biotechnology , biology , biochemistry , genetics
An experimental study has been carried out to investigate the effectivenes of several reduced serum and serum‐free media for the cultivation of an ovarian cell line, Bm5, of the lepidopteran insect Bombyx mori. Bm5 cell were successfully adapted to grow in a medium containing 5% serum and a serum‐free medium (EX‐CELL 400). On the other hand, this cell line could not be adapted to grow in several other media suggested in the literature, including IPL‐41 + 2% fetal bovine serum (FBS), SF‐900, and a serum‐free medium (ISFM). Furthermore, a comparative study was conducted to determine the production levels of B. mori nuclear polyhedrosis virus (BmNPV) in Bm5 cells cultured in three different medium formulations. The production levels of BmNPV in adapted Bm5 cells grown in a 5% serum‐supplemented medium and a serum‐free medium (EX‐CELL 400) were comparable to those obtained in Bm5 cells grown 10% serum‐supplemented medium. © 1992 John Wiley & Sons, Inc.