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Determination of intraparticle immobilized enzyme distribution under moderate diffusion conditions
Author(s) -
Hossain Md. M.,
Do D. D.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260400614
Subject(s) - diffusion , chemistry , immobilized enzyme , chromatography , enzyme , distribution (mathematics) , biochemistry , thermodynamics , mathematics , physics , mathematical analysis
The method Presented earlier by Hossain and Do determine the active enzyme distribution and relevant rate parameters under the condition of strong diffusional resistance is extended in this article to cover the cases of comparable diffusion and reaction rates (3 < ϕ < 20). The theory proposed herein is tested wtih the experimental data of hydrogen perioxide‐catalase immobilized on controlled‐pore glass (CPG) particles of small size (150 μm). © 1992 John Wiley & Sons, Inc.