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Iridium oxide pH microelectrode
Author(s) -
VanHoudt Paulus,
Lewandowski Zbigniew,
Little Brenda
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260400507
Subject(s) - iridium , microelectrode , platinum , electrode , chemistry , oxide , sulfuric acid , inorganic chemistry , analytical chemistry (journal) , chemical engineering , chromatography , catalysis , organic chemistry , engineering
The manufacture, calibration, and signal conditioning during construction of an iridium/iridium oxide pH microsensor is described. The microsensor was designed to be used extracellularly, primarily in biofilm research. The sensing tip diameters were typically in the range of 3–15 μm. The iridium oxide was formed by potential cycling in dilute sulfuric acid. A pH profule across a denitrifying biofilm was measured as an example of an application. The higher Nernstian slope (70–80 mV/pH for fresh electrodes), increased rigidity, and restriction of the sensing tip to the outermost end of the electrode are features which make the iridium/iridium oxide pH microelectrode superior to a glass microelectrode. © 1992 John Wiley & Sons, Inc.