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A structured dynamic model for the baculovirus infection process in insect‐cell reactor configurations
Author(s) -
De Gooijer Cornelis D.,
Koken Rick H. M.,
Van Lier Frank L. J.,
Kool Marcel,
Vlak Just M.,
Tramper Johannes
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260400413
Subject(s) - autographa californica , nuclear polyhedrosis virus , bioreactor , spodoptera , baculoviridae , virus , biology , biological system , viral infection , virology , insect , genetics , gene , botany , recombinant dna
Abstract A mathematical description of the infection of insect cells with baculovirus in a continuously operated reactor configuration is presented. The reactor configuration consists of one bioreactor in which insect cells ( Spodoptera frugiperda ) are grown followed by one or two bioreactors in which cells are infected by a baculovirs ( Autographa californica nuclear polyhedrosis virus). It was demonstrated that the so‐called passage effect is responsible for the observed difference in run time between a configuration with one or with two infection vessels. Furthermore, a model is presented based on the hypothesis that the limited run time of series of continuously operated bioreactors is associated with the occurrence of a virus particle (so‐called virion) that is defective and has interfering properties. With the assumption that not all nonoccluded virions are capable of establishing a correct infection leading to new virus production, infection levels in continuously operated reactor configurations could be described well with the model. © 1992 John Wiley & Sons, Inc.