Premium
How to predict product yield in kinetically controlled enzymatic peptide synthesis
Author(s) -
Gololobov Mikhail Yu.,
Kozlova Elena V.,
Borisov Ivan L.,
Schellenberger Ute,
Schellenberger Volker,
Jakubke HansDieter
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260400313
Subject(s) - yield (engineering) , papain , peptide , chemistry , enzyme , combinatorial chemistry , peptide synthesis , beta (programming language) , stereochemistry , biochemistry , computer science , thermodynamics , physics , programming language
The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters α and β for papain‐catalyzed synthesis of Mal‐ L ‐Phe‐ L ‐Ala‐ L LeuNH 2 from Mal‐ L ‐Phe‐ L ‐AlaOMe and L ‐LeuNH 2 . The experimental results demonstrate that this theory adequately describes the real process. © 1992 John Wiley & Sons, Inc.