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Cell cycle model for growth rate and death rate in continuous suspension hybridoma cultures
Author(s) -
Linardos Theodor I.,
Kalogerakis Nicolas,
Behie Leo A.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260400305
Subject(s) - steady state (chemistry) , flow cytometry , cell cycle , growth rate , biology , cell culture , cell growth , suspension culture , suspension (topology) , cell division , programmed cell death , biophysics , cell , chemistry , microbiology and biotechnology , biochemistry , apoptosis , genetics , geometry , mathematics , homotopy , pure mathematics
As a result of recent advances in flow cytometry, renewed interest is shown in modeling the kinetic behavior of cells in culture on the basis of cell cycle parameters. An important but often overlooked kinetic variable in hybridoma cultures is the cell death rate. Not only the overall cell growth but also the kinetics of nutrient metabolism and monoclonal antibody production have been shown to depend on the cell death rate in continuous suspension hybridoma cultures. The present study shows that the death rate in hybridoma cultures is proportional to the fraction of cells arrested in the G 1 phase of the cell cycle. The steady‐state cell age distributions in the various phases of the division cycle have been calculated analytically. A simple mathematical model has been used to produce the profiles of the cycling and arrested cell fractions with respect to the dilution rate. The calculated steady‐state growth rate, death rate, and viability profiles are shown to be in agreement with recently published experimental data from continuous suspension hybridoma cultures. © 1992 John Wiley & Sons, Inc.