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Effect of low serum concentrations (0%–2.5%) on growth, production, and shear sensitivity of hybridoma cells
Author(s) -
van der Pol L.,
Bakker W. A. M.,
Tramper J.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260400125
Subject(s) - monoclonal antibody , sparging , cell culture , chemistry , chromatography , bubble , bubble column reactor , lysis , biology , biophysics , microbiology and biotechnology , antibody , immunology , mechanics , physics , genetics , gas bubble
In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody porduction have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable‐cell concentrations, but cell specific monoclonal‐antibody production rate shows a decreasing trend. A gradual increase in sensitivity for sparging, which is nor the result of a long‐term biological effect, has beeen measured in a bubble column at decreasing fetal calf serum concentrations. Finally, the hypothetical killing‐volume model describing the death rate of insect cells in bubble columns has now been completely validated for the pertinent hybridoma‐cell line.