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The effects of surface adsorption on the thermal stability of proteins
Author(s) -
Steadman Bryan L.,
Thompson Karen C.,
Middaugh C. Russell,
Matsuno Ken,
Vrona Susan,
Lawson Erlinda Q.,
Lewis Randolph V.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260400103
Subject(s) - adsorption , thermal stability , differential scanning calorimetry , protein adsorption , chemistry , lysozyme , fourier transform infrared spectroscopy , surface protein , chemical engineering , organic chemistry , biochemistry , thermodynamics , physics , virology , biology , engineering
The effect of surface adsorption on the structure and stability of proteins is a matter of increasing interest in biotechnology. Therefore, we have examined the effect of adsorption to silica on the thermal stability of 7 proteins employing differential scanning calorimetry (DSC) and front surface fluorescence (FSF) spectroscopy. In general, it was found that surface adsorption decreased the thermal stability of the bound protein. Using lysozyme for further studies, DSC, FSF, and FTIR spectroscopies, as well as enzymatic activity measurements, were used to explore the effect of decreasing surface apolarity on stability. It was observed that increasing surface apolarity produced decreasing stability and increasing structural alteration of the adsorbed protein.