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A fluorescence‐based fiber optic biosensor for the flow‐injection analysis of penicillin
Author(s) -
Xie Xiangfang,
Suleiman Ahmad A.,
Guilbault George G.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260391111
Subject(s) - penicillin , flow injection analysis , optical fiber , fluorescein isothiocyanate , biosensor , chemistry , fluorescence , membrane , fiber optic sensor , chromatography , fiber , biochemistry , detection limit , antibiotics , optics , organic chemistry , physics
A penicillin fiber optic sensor is described. The sensor is based on co‐immobilization of a pH indicator, fluorescein isothiocyanate (FITC), and penicillinase on a preactivated biodyne B membrane attached to the end of a bifurcated optical fiber. The characteristics of the sensor are investigated in conjunction with a flow injection analysis system. The proposed sensor is reversible and responds to penicillin in the concentration range of 1 × 10 −4 to 5 × 10 −2 mol/L. The application of this sensor to penicillin analysis in some pharmaceutical samples is demonstrated.