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Alkaloid production by plant cell suspension cultures of Holarrhena antidysenterica : I. Effect of major nutrients
Author(s) -
Panda A. K.,
Mishra Saroj,
Bisaria V. S.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260391008
Subject(s) - alkaloid , kinetin , apocynaceae , chemistry , sucrose , nutrient , murashige and skoog medium , dry weight , botany , acetic acid , biology , food science , tissue culture , in vitro , biochemistry , organic chemistry
The effect of major nutrients on growth and alkaloid production by plant cell culture of Holarrhena antidysenterica was studied with a view to increasing the yield of the alkaloid conessine, a therapeutic drug used for treatment of dysentery and helminthic disorders. The studies resulted in development of a modified Murashige and Skoog (MS) medium that contained 60 m M total nitrogen with a NH 4 + ‐to‐NO 3 − ratio of 5:1, 0.25 m M phosphate, and 40 g/L sucrose. The growth regulators 2,4‐dichlorophenoxy acetic acid (2,4‐D) and kinetin (Kn) were also found to affect the synthesis of alkaloid. Using an optimal level of inoculum (3 g/L), the modified medium resulted in alkaloid synthesis of 0.66 g/100 g dry cell weight, which represented a 4.25‐fold increase over that obtained in standard MS medium.