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Effect of serum concentration on hybridoma viable cell density and production of monoclonal antibodies in cstrs and on shear sensitivity in air‐lift loop reactors
Author(s) -
Martens D. E.,
Beuvery E. C.,
De Gooijer C. D.,
Tramper J.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260390902
Subject(s) - monoclonal antibody , bioreactor , shear (geology) , lift (data mining) , chemistry , sensitivity (control systems) , materials science , antibody , biology , immunology , engineering , composite material , computer science , organic chemistry , electronic engineering , data mining
The death rate of hybridoma cells, grown in a continuous culture, has been studied in a small air‐lift loop reactor as a function of reactor height and injected gas flow rate. The first‐order death‐rate constant was found to be proportional to the reciprocal height and to the gas flow rate, in accordance with the hypothetical killing volume model for insect cells in bubble columns. Furthermore, the effect of the serum concentration on viable cell concentration and cell productivity has been investigated in a continuous culture. A serum component became growth limiting when the serum concentration was decreased from 2% to 1%. No effect of the serum concentration on specific cell productivity could be measured. Samples from this culture were also studied in the air‐lift loop reactor to determine the effect of serum concentration on the shear sensitivity. The cells' shear sensitivity increased with decreasing serum concentration. The protective effect of serum was found to be physical as well as physiological.