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Production of superoxide dismutase in Streptococcus lactis by a combination of use of hyperbaric oxygen and fermentation with cross‐flow filtration
Author(s) -
Taniguchi Masayuki,
Hoshino Kazuhiro,
Itoh Takehiro,
Kumakura Hiroshi,
Fujii Michihiro
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260390811
Subject(s) - superoxide dismutase , catalase , fermentation , food science , chemistry , hydrogen peroxide , biochemistry , microbiology and biotechnology , anaerobic exercise , chromatography , biology , antioxidant , physiology
The production conditions of superoxide dismutase (SOD) in the cells of Streptococcus lactis by using hyperbaric oxygen (O 2 ) are described. The SOD activity of anaerobically grown cells was 5–6 U/mg protein. When the culture broth was pressurized by O 2 at 6 atm, the SOD activity was more than twice as high as that under anaerobic culture conditions. However, there is little or no significant increase in SOD activity by exogenous addition of catalase for detoxifying hydrogen peroxide accumulated in the broth and/or controlling the pH of the broth at 6.8 during the pressurization by O 2 . The increase in SOD activity by hyperbaric O 2 was possible not only at the late‐logarithmic growth phase but also at the initial time for the stationary growth phase. For improvement of SOD productivity, we tried a two‐stage culture in which SOD activity in S. lactis cells was enhanced by pressurizing the culture broth using hyperbaric O 2 after achievement of a high‐concentration cultivation in the anerobic fermentation system with a microfiltration module.