Premium
Growth of the obligate methylotroph Methylobacillus flagellatum under stationary and nonstationary conditions during continuous cultivation
Author(s) -
Baev M. V.,
Schklyar N. L.,
Chistoserdova L. V.,
Chistoserdov A. Y.,
Polanuer B. M.,
Tsygankov Y. D.,
Sterkin V. E.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260390614
Subject(s) - chemostat , obligate , methanol , growth rate , biology , steady state (chemistry) , bacteria , pulse (music) , chemistry , microbiology and biotechnology , botany , mathematics , genetics , physics , geometry , organic chemistry , detector , optics
The growth characteristics of a chemostat culture of the obligate methylotrophic bacterium Methylobacillus flagellatum have been determined. Steady‐state cultures growing at a rate of 0.73–0.74 h −1 , equal to the maximal growth rate, were obtained under oxyturbidostat cultivation conditions. The response of a chemostat culture to a pulse increase of methanol concentration was studied. It was shown that slow and rapidly growing cultures of M. flagellatum responded differently to pulse methanol addition. The growth characteristics of slow‐growing cultures decreased after methanol addition compared to those of stationary chemostat cultures. The growth characteristics of rapidly growing cultures were practically unchanged with and without pulse methanol addition.