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Mathematical modeling of induced foreign protein production by recombinant bacteria
Author(s) -
Lee Jongdae,
Ramirez W. Fred
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260390608
Subject(s) - recombinant dna , bacteria , production (economics) , biochemical engineering , chemistry , microbiology and biotechnology , biology , biochemistry , engineering , economics , genetics , gene , macroeconomics
A new generalized mathematical model for recombinant bacteria which includes inducer effects on cell growth and foreign protein production is developed. The model equation set was applied to a host‐vector system, Escherichi coli D1210 and plasmid pSD8. Batch experiments were designed and performed in shake flasks to verify the model. A parameter estimation method was developed and proven to be efficient. Although simple, the model can effectively describe the dynamics of the production of foreign protein in recombinant bacteria and can be used for optimization and control studies to maximize foreign protein production.