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Quantification of cell culture factors affecting recombinant protein yields in baculovirus‐infected insect cells
Author(s) -
Lindsay David A.,
Betenbaugh Michael J.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260390605
Subject(s) - aeration , recombinant dna , cell culture , cell , biology , baculoviridae , chromatography , biochemistry , chemistry , food science , ecology , gene , genetics , spodoptera
An experimental study was undertaken to quantify the effects of infection cell density, medium condition, and surface aeration on recombinant protein yields in insect cells. In the absence of surface aeration and fresh medium, insect cells generated higher product yields (on a per cell basis) when infected with recombinant baculovirus at low cell densities, LCD (3 × 10 5 −4 × 10 5 cells/mL), than at high cell densities, HCD (>0.9 × 10 6 cells/mL), for two distinct baculovirus types. Surface aeration of a HCD culture infected in spent medium improved β‐glactosidase yields 5‐fold over the nonaerated case. Surface aeration and medium replenishment improved β‐galactosidase yields of a HCD culture by 20‐fold (compared to a 1.6‐fold improvement for a LCD culture), resulting in cultures with productivties that were independent of the cell density at infection.