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Displacement effects in large‐scale chromatography?
Author(s) -
Jungbauer Alois,
Uhl Karola,
Schulz Petra,
Tauer Christa,
Gruber Gerhard,
Steindl Franz,
Buchacher Andrea,
Schoenhofer Wolfgang,
Unterluggauer Florian
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260390514
Subject(s) - elution , chemistry , chromatography , displacement (psychology) , resolution (logic) , displacement chromatography , volume (thermodynamics) , ion chromatography , biomolecule , gradient elution , analytical chemistry (journal) , column chromatography , high performance liquid chromatography , chromatography column , biochemistry , thermodynamics , physics , psychology , artificial intelligence , computer science , psychotherapist
Displacement effects in large‐scale (total column volume v t = 150 L) and preparative ion‐exchange chromatography purifying human erythrocyte superoxide dismutase are described in the present article. The biomolecules are eluted in a very small peak elution volume (<0.2 v o ) behind the salt wave using a step gradient. The theoretical peak width and retention behavior are calculated according to the model of Yamamoto. The theoretical values are then compared with the experimental data. There was a difference observed between the elution type I (also called fronting) and the experimentally obtained elution. Some instructions are given on how to achieve these phenomenona because a beneficial effect in respect to resolution and recovery of a biomolecule is observed.
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