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Glucose‐limited chemostat culture of chinese hamster ovary cells producing recombinant human interferon‐γ
Author(s) -
Hayter Paul M.,
Curling Elisabeth M. A.,
Baines Anthony J.,
Jenkins Nigel,
Salmon Ian,
Strange Phillip G.,
Tong Jeremy M.,
Bull Alan T.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260390311
Subject(s) - chinese hamster ovary cell , chemostat , recombinant dna , cell culture , biology , glycosylation , biochemistry , yield (engineering) , hamster , interferon , medicine , endocrinology , bacteria , immunology , genetics , materials science , metallurgy , gene
A Chinese hamster ovary (CHO) cell line expressing recombinant human interferon‐γ (IFN‐γ) was grown under glucose limitation in a chemostate at a constant dilution rate of 0.015 h −1 with glucose feed concentrations of 2.75 m M and 4.25 m M. The changes in cell concentration that accompanied changes in the glucose feed concentration indicated that the cells were glucose‐limited. The cell yield on glucose remained constant, but there was a decline in residual glucose concentration and a reduced lactate yield from glucose in the latter stages of the culture. The consumption rates for many of the essential amino acids were increased later in the culture. The volumetric rate of interferon‐γ production was maintained throughout the course of this culture, indicating that IFN‐γ expression was stable under these conditions. However, the specific rate of IFN‐γ production was significantly lower at the higher glucose feed concentration. Under glucose limitation, the proportion of fully glycosylated IFN‐γ produced by these cells was less than that produced in the early stages of batch cultures. The proportion of fully glycosylated IFN‐γ increased during transient periods of glucose excess, suggesting that the culture environment influences the glycosylation of IFN‐γ.