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Characterization of pellet morphology during submerged growth of Streptomyces tendae by image analysis
Author(s) -
Reichl U.,
King R.,
Gilles E. D.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260390207
Subject(s) - pellet , pellets , yield (engineering) , fermentation , mycelium , morphology (biology) , characterization (materials science) , streptomyces , biological system , materials science , chemistry , chromatography , chemical engineering , bacteria , biology , food science , botany , composite material , nanotechnology , genetics , engineering
Many filamentous bacteria and fungi tend to form pellets, or mixtures of dispersed mycelium and pellets in liquid fermentation broths. In some cases, a specific kind of morphology is required for optimum product yield. When quantitative analysis and characterization of the pellet morphology are needed, an image processing system can be used. It allows a fast and reproducible analysis of the frequency distribution of pellet size, mean pellet size, contents of pellets, or their shape. The use of such a system allows for an on‐line analysis. For a demonstration of the method, results of two fermentations of Streptomyces tendae are shown.