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In vitro culture of hybridoma cells in agarose beads producing antibody secretion for two weeks
Author(s) -
Cadic Ch.,
Dupuy B.,
Pianet I.,
Merle M.,
Margerin Ch.,
Bezian J. H.
Publication year - 1992
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260390115
Subject(s) - agarose , microbead (research) , cell culture , in vitro , antibody , chromatography , chemistry , suspension (topology) , microbiology and biotechnology , secretion , sepharose , biochemistry , biology , immunology , enzyme , genetics , mathematics , homotopy , pure mathematics
A new process for embedding cells in agarose is described. Beads were obtained by extruding an ultralow gelling temperature agarose solution in a capillary containing a hydrophobic medium flowthrough. The toxicity of the procedure has been evaluated by monitoring the energy status of agarose‐embedded C 6 glioma cells with 31 P nuclear magnetic resonance (NMR). Suspension and microbead cultures of hybridoma cell line were compared. In suspension culture the number of cells and the antibody concentrations increased for 5 days before the stationary phase began, when the cultures were stopped. In agarose bead cultures, the gel provided an enormous support surface area (50 m 2 / mL of gel). It was possible to seed 20‐fold more cells. The gel pressure modified the proliferative process and antibody pattern secretion. In particular, the antibodies could be harvested for two weeks.