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Selective separation and purification of two lipases from chromobacterium viscosum using AOT reversed micelles
Author(s) -
AiresBarros M. R.,
Cabral J. M. S.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260381107
Subject(s) - lipase , chromatography , chemistry , potassium phosphate , extraction (chemistry) , aqueous solution , triacylglycerol lipase , micelle , phosphate , potassium , aqueous two phase system , enzyme , organic chemistry
Selective separation and purification of two lipases form Chromobacterium viscosum were carried out by liquid‐liquid extraction using a reversed micellar system. Optimum parameters for extraction were determined using a 250 m M AOT micellar solution in isooctane. Complete separation of the two lipases was achieved at pH 6.0 with a 50m M potassium phosphate buffer solution containing 50 m M KCI. By adding 2.5% by volume of ethanol to the lipase‐loaded micellar solution, 85% of the extracted lipase could be recovered in a new aqueous phase, 50 m M K 2 HPO 4 with 50 m M KCl, at pH 9.0. Lipase A was purified 2.6‐fold with a recovery of 86%, and lipase B by 1.5‐fold with a recovery of 76%.