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Modeling of hollow‐fiber capillary reactor for the production of L ‐Alanine with coenzyme regeneration
Author(s) -
Fujii Tomoyuki,
Miyawaki Osato,
Yano Toshimasa
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260381008
Subject(s) - nad+ kinase , alanine , bioreactor , chemistry , lactate dehydrogenase , biochemistry , dehydrogenase , alcohol dehydrogenase , chromatography , substrate (aquarium) , enzyme , biology , amino acid , organic chemistry , ecology
Abstract Continuous production of L ‐alanine with conjugated enzyme systems of alanine dehydrogenase (AlaDH) and lactate dehydrogenase (LDH) or alcohol dehydrogenase (ADH) was carried out with NAD regeneration in an ultrafiltration hollow‐fiber capillary reactor (HFCR) which was proposed as a test bioreactor with very small scale. In the AlaDH/LDH system, pyruvate is the intermediate product for L ‐alanine so that an optimal point existed in pyruvate concentration for the production rate of L ‐alanine. NAD cycling number of 4850 and L ‐alanine productivity of 61.7 mmol/L h were obtained at the best condition. In the AlaDH/ADH system, however, the substrate inhibition in the AlaDH reaction by pyruvate should be considered and the best results of NAD cycling number and L ‐alanine productivity were 2700 and 13.5 mmol/L h, respectively. In consideration of concentration distribution and mixing in the axial direction on an HFCR, performance of the reactor was theoretically analyzed with a multistage stirred tank reactor model combined with the kinetic model based on all the elementary reactions involved. Although quantitative discrepancy existed in some cases, the present theoretical model could explain experimental results and is expected to be generally applicable to standard hollow fiber reactors.