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Continuous protein separations in a magnetically stabilized fluidized bed using nonmagnetic supports
Author(s) -
Chetty Ashok S.,
Burns Mark. A.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260380902
Subject(s) - fluidization , adsorption , lysozyme , chromatography , mass transfer , myoglobin , fluidized bed , aqueous solution , chemistry , packed bed , volume (thermodynamics) , magnetic field , chemical engineering , materials science , analytical chemistry (journal) , thermodynamics , organic chemistry , biochemistry , physics , quantum mechanics , engineering
Abstract Continuous protein separations were performed using a magnetically stabilized fluidized bed (MSFB) and a commercially available affinity adsorption resin that contained no magnetically susceptible material. These nonmagnetic materials can be stabilized at relatively low fields (<75 G requiring <30 W) if sufficient magnetically susceptible particles are also present in the stabilized bed. The minimum amount of magnetic particles necessary to stabilize the bed is as low as 20% by volume and is a function of various parameters including the size and density of both particles, the magnetic field strength, and the fluidization velocity. Advantages of these beds for performing separations include true continuous, countercurrent liquid‐solids contact, mass‐transfer efficiencies nearly equal to that of packed beds, and the ability of handle suspended cells or cell debris. A variety of commercially available affinity, ion‐exchange, and adsorptive supports can be used in the bed for continuous separations; results are presented for the adsorption and recovery of lysozyme from an aqueous mixture of lysozyme and myoglobin using an affinity resin.