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Synthesis and secretion of α‐amylase by rice callus: Evidence for differential gene expression
Author(s) -
Simmons Carl R.,
Huang Ning,
Cao Yicheng,
Rodriguez Raymond L.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260380513
Subject(s) - amylase , secretion , callus , gene expression , gene , biology , microbiology and biotechnology , chemistry , biochemistry , enzyme , botany
Rice seed callus expressed and secreted α‐amylase at high levels. Twenty percent of the protein secreted by the callus was αamylase. The callus secreted about 840 μg α‐amylase with 10.9 × 10 3 units of activity per gram dry weight callus per day. The α‐amylase from callus exhibited a more complex isoform pattern than the germinating seed α‐amylase. In addition, the level of mRNA expression by the five α‐amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which it attractive as a potential system for expression proteins in plant cell fermentation systems.