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Growth and substrate consumption of Nitrobacter agilis cells immobilized in carrageenan: Part 2. Model evaluation
Author(s) -
Wijffels R. H.,
de Gooijer C. D.,
Kortekaas S.,
Tramper J.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260380304
Subject(s) - airlift , substrate (aquarium) , biomass (ecology) , chromatography , carrageenan , oxygen , chemistry , limiting , chemical engineering , bioreactor , biochemistry , biology , ecology , organic chemistry , mechanical engineering , engineering
A dynamic model that predicts substrate and biomass concentration profiles across gel beads and from that the overall substrate consumption rate by the gel beads containing growing cells was evaluated with immobilized Nitrobacter agilis cells in an airlift loop reactor with oxygen as the limiting substrate. The model predictions agreed well with the observed oxygen consumption rates at three different liquid phase oxygen concentrations. Image analysis showed that 90% of the immobilized cells after 42 days of cultivation was situated in the outer shells in a film of 140 μm, while the bead radius was about 1 mm. The maximum biomass concentration in the outmost film of 56 μm was 11 kg · m −3 gel.