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NADH fluorscence in a carbon‐limited fermentation
Author(s) -
Srivastava A. K.,
Volesky B.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260380211
Subject(s) - dilution , fermentation , chemistry , solvent , sugar , acetone , butanol , population , carbon fibers , chromatography , food science , ethanol , biochemistry , thermodynamics , materials science , physics , demography , sociology , composite number , composite material
Abstract The continuous flow acetone–butanol fermentation conducted at lowered inlet feed sugar concentrations and at a constant dilution rate D =0.075 h −1 demonstrated a significant decreases in the availability of the cell population “reduction energy” (F/X), resulting in an exclusive accumulation of intermediate acids under those conditions. The cultures resumed its solvent production activity when the inlet sugar concentration in the feed stream was increased from 20 to 40 g/L at the same low growth/dilution rates. A linear correlation between the culture reduction content (F/X) and the specific butanol rate ( q B ) was observed under the present conditions, indicating the necessity of the NADH availability for the increased solvent production.