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Modeling growth and succinoglucan production by Agrobacterium radiobacter NCIB 9042 in batch cultures
Author(s) -
Dussap C. G.,
De Vita D.,
Pons A.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260380109
Subject(s) - aeration , nitrogen , oxygen , biomass (ecology) , chemistry , bioreactor , cofactor , stoichiometry , biochemistry , continuous stirred tank reactor , exponential growth , polysaccharide , metabolism , food science , chromatography , biology , organic chemistry , enzyme , ecology , mathematical analysis , mathematics
Wild‐type Agrobacterium radiobacter NCIB 9042 has been cultivated in batch cultures on a synthetic medium which was adapted for growth and succinoglucan production. Experiments were carried out in a 4‐L stirred‐tank aerated reactor. Glucose, biomass, polysaccharide, protein, and inorganic‐ and organic‐nitrogen concentrations were measured, and oxygen consumption and CO 2 production rates were obtained by a gas‐balance technique. Nitrogen balance shows that inorganic nitrogen is entirely recovered into proteins. The carbon balance is satisfied with in ±5%. Stoichiometric equations for biomass growth and succinoglucan synthesis were established. The biosyntheticpolymer pathways including ATP and cofactor consumption were investigated. From previous studies, a (P/O) value of 1.66 is selected for oxygen sufficient cultures. The actual ATP requirements of 25.4 mmol ATP/g succinoglucan (38.5 mol ATP/mol succinoglucan), determined by a metabolic analysis, is 2.39 times the stoichiometric value. Experimental results were modeled by a system of differential equations. The exponential growth phase was described by a nitrogen‐limited Monod equation. Subsequent succinoglucan synthesis followed a slightly modified Luedeking–Piret relation partitioning internal and external polysaccharide. Experimentally determined coefficients are compared with published results for continuous culture of A. radiobacter NCIB 11883.