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Oxygen uptake by entrapped hybridoma cells
Author(s) -
Wohlpart Dave,
Gainer John,
Kirwan Donald
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260371110
Subject(s) - oxygen , calcium alginate , suspension culture , chemistry , suspension (topology) , calcium , cell culture , biophysics , cell , limiting oxygen concentration , biochemistry , chromatography , biology , mathematics , organic chemistry , homotopy , pure mathematics , genetics
Oxygen consumption by hybridoma cells immobilized in 1‐ and 3.9‐mm‐diameter calcium alginate beads was measured. The entrapped cells consumed oxygen at about 10 μmol/min per 10 9 cells, regardless of the bead size and cell loading. In contrast, the same cells in suspension culture respire at specific rates of 3–8 μmol/min per 10 9 cells (depending on the cell density). The growth rate of the immobilized cells was significantly reduced, while specific antibody production was comparable to that of free cells.