Premium
Use of fluorometry for monitoring and control of a bioreactor
Author(s) -
Li JainKe,
Humphrey Arthur E.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260371109
Subject(s) - bioreactor , fluorescence spectroscopy , chemistry , fluorescence , biochemical engineering , chromatography , engineering , physics , optics , organic chemistry
A new fluorescent bioreactor monitoring probe—multiple excitation fluorometric system (MEFS)—has been developed. This probe was compared to the commercially available BioChem Technology FluroMeasure system (NADH probe). In this task the fluorescence behavior of three model fermentation systems, ethanol fermentation by Candida utilis , phenol fermentation by Pseudomonas putida , and glucose fermentation by Saccharomyces cerevisiae , were examined. The results indicated that the fluorescence intensity and behavior of various cellular fluorophors vary significantly between the different fermentation systems. Monitoring a fermentation process using only NAD(P)H fluorescence provided limited information. The NAD(P)H fluorescence was found not to be the best fluorescence signal for monitoring cell concentrations. The best way of monitoring a bioreactor by fluorometry may be to monitor several fluorophors in the whole culture broth simultaneously and to relate these fluorescence signals to various biological parameters.