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Transient response method for evaluating the rate constants of reactions over immobilized enzymes
Author(s) -
Wu JauYann,
Weng HungShan
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260371005
Subject(s) - glucose oxidase , diffusion , immobilized enzyme , chemistry , reaction rate constant , michaelis–menten kinetics , transient (computer programming) , transient response , reaction rate , enzyme , reaction–diffusion system , chromatography , catalysis , constant (computer programming) , thermodynamics , dispersion (optics) , analytical chemistry (journal) , kinetics , organic chemistry , enzyme assay , physics , electrical engineering , quantum mechanics , computer science , engineering , operating system , programming language , optics
The transient response method was utilized to evaluate the rate constants of reaction over immobilized enzyme. Glucose oxidation catalyzed by the immobilized glucose oxidase in a fixed‐bed reactor was selected as an example. A theoretical model including the effects of axial dispersion, film diffusion, and intraparticle diffusion was established for the reactor. The individual rate constant of each elementary step of this enzymatic reaction was determined through direct fitting of the experimental response data to the model.