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Photosynthetic activity of plant cells solubilized in water‐in‐oil microemulsions
Author(s) -
Hochkoeppler A.,
Luisi P. L.
Publication year - 1991
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260371004
Subject(s) - microemulsion , photosynthesis , pulmonary surfactant , oxygen , chemistry , solubilization , aqueous solution , plant cell , botany , biochemistry , organic chemistry , biology , gene
With the aim of possibly extending plant microbiology and photosynthesis beyond the usual applicability in aqueous solution, we investigated the solubilization of plant cells inorganic media with the help of water‐in‐oil microemulsions. Cells isolated from leaves of Rumex obtusifolius were solubilized in a water/2‐ethyl‐hexyl‐sodiumsulfosuccinate/isooctane system, containing 20% water (v:v) and 240 m M surfactant, and the oxygen evolution/consumption was measured polarographically. Although no oxygen evolution was detectable in the organic medium, the cells were able to carry out photosynthetic oxygen consumption at the expense of ascorbate. To a lesser extent, photosynthetic oxygen consumption was measured using N , N , N′ , N′ ‐tetramethyl‐ p ‐phenylenediamine as electron donor. The rate of ascorbate photooxidation was linearly related to the concentration of cells.